塔里木大学学报

本实验通过对玉米gif1突变体与其野生型在株型、光合生理性状的研究比较分析，发现玉米ZmGIF1基因在调控叶片的空间分布及株型上具有一定的影响；gif1突变体株高、穗位高度、穗位叶长、穗位叶宽等与野生型差异极显著，说明ZmGIF1基因可能会导致植株基因功能的下降，甚至会丧失；gif1突变体不同部位、不同时期的叶绿素含量尤其在拔节期后显著低于野生型；净光合速率显著低于野生型植株，说明ZmGIF1基因可能影响玉米叶绿素的形成以及光合产物的积累；gif1突变体植株的叶片宽度较窄，但叶片较为平展，呈紧凑对生状态，通风透光性不如野生型好。gif1突变体的蒸腾速率、气孔导度及水汽压亏缺下降，表明gif1突变体光合产物积累少，但在高温、干旱的情况下，通过降低气孔导度、蒸腾速率以及减少水汽压亏缺等方式一定程度地提高抗旱性，说明ZmGIF1基因对研究株型结合光合生理以及抗旱性育种是一个有益的基因资源。

In this study, the plant type and photosynthetic physiological characters of gif1 mutant and its wild type were studied.The results showed that ZmGIF1 gene had a certain effect on regulating the spatial distribution and plant type of leaves. The plant height,spike height, spike leaf length, spike leaf width of gif1 mutant were extremely significantly different from those of the wild type, explaining that ZmGIF1 gene may lead to the decline of plant gene function or even loss. The chlorophyll content in different parts and periods of gif1 mutant was significantly lower than that of wild type, especially after jointing stage. The net photosynthetic rate was dramatically lower than that of wild type plants, illustrating that ZmGIF1 gene may affect the formation of chlorophyll and the accumulation of photosynthetic products in maize. The leaf width of the gif1 mutant plant was narrow, but the leaf was relatively flat,shown a compact opposite state, and the ventilation and light transmittance was not as good as that of the wild type. The transpiration rate, stomatal conductance and water vapor pressure deficit of gif1 mutant decreased, indicating that the accumulation of photosynthetic products in gif1 mutant was less. However, under the conditions of high temperature and drought, the drought resistance of gif1 mutant was improved to a certain extent by reducing stomatal conductance, transpiration rate and water vapor pressure deficit,manifesting that ZmGIF1 gene is a useful gene resource for studying plant type binding photosynthetic physiology and drought resistance breeding.