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为了观察自制紫草抑菌膏对Ⅱ度烧伤小鼠背部皮肤创面愈合的效果以及对Ⅰ、Ⅲ型胶原和透明质酸标志物CD44含量变化的影响，验证其药效。试验使用27只8周龄昆明小鼠，通过使用烧烫伤造模仪在100℃下烫伤背部皮肤5 s,建立Ⅱ度烧伤模型。将小鼠分为3组：空白对照组、疗效对照组(市售某烧烫伤膏)和药物治疗组(紫草抑菌膏组),每隔1 d给药，分别在第3 d、第9 d和第13 d取小鼠背部皮肤样本进行天狼星红染色、Masson染色以及免疫荧光染色，以观察Ⅰ型、Ⅲ型胶原含量、总胶原蛋白含量及透明质酸含量的变化。结果表明：药物治疗组中的Ⅰ型胶原含量随烧伤时间增长而下降，Ⅲ型胶原含量高于空白对照组和疗效对照组；在第3 d、第9 d和第13 d,药物治疗组的总胶原蛋白含量均高于空白对照组和疗效对照组。CD44的平均荧光强度在3组中均呈先增后减的趋势，且药物治疗组在第3 d、第9 d和第13 d的平均荧光强度均高于空白对照组和疗效对照组。试验结果说明，紫草抑菌膏可以促进透明质酸增多，进而促进基底层细胞的增殖、分化和迁移，加快烧伤皮肤的愈合。其还能促进Ⅲ型胶原在基底层的沉积，增加Ⅲ型胶原比例，减少瘢痕组织，药效优于疗效对照组。
Abstract：In order to observe the effect of Arnebia euchroma antibacterial ointment on the healing of skin wounds on the back of Ⅱ degree burn mice and verify its efficacy, 27(8-week-old) Kunming mice were used in the experiment. A second-degree burn model was established by scalding the back skin at 100 ℃ for 5 seconds using a digital electronically controlled scald instrument. After 15 days of culture, the mice were divided into three groups: blank control group, therapeutic effect control group(a certain burn and scald ointment is available on the market)and drug treatment group(Arnebia euchroma antibacterial ointment group). Every other day, the skin samples of the back of the mice were taken on the 3rd, 9th and 13th days for Sirius red staining, Masson staining and immunofluorescence staining to observe the changes of type Ⅰ and type Ⅲ collagen content, total collagen content and hyaluronic acid content. Results showed that the content of type Ⅰ collagen in the shikonin antibacterial ointment group decreased with time, and the content of type Ⅲ collagen was higher than that in the blank control group and the therapeutic effect control group. On the 3rd, 9th and 13th day, the total collagen content of the drug treatment group was higher than that of the blank control group and the therapeutic effect control group. The average fluorescence intensity of CD44 showed a trend of increasing first and then decreasing in the three groups, and the average fluorescence intensity of drug treatment group was higher than that of blank control group and therapeutic effect control group on the 3rd, 9th and 13th day. It shows that drug treatment group can promote the increase of hyaluronic acid, and then promote the proliferation, differentiation and migration of basal layer cells, and accelerate the healing of burn skin. It can also promote the deposition of type Ⅲ collagen in the basal layer, increase the proportion of type Ⅲ collagen, reduce scar tissue, and its efficacy is better than that of therapeutic effect control group.
[1] 许行.地龙木耳散对大鼠深Ⅱ度烫伤创面修复作用的初步研究[D].青岛：青岛大学，2022.
[2] 许景江，张轶星，蓝公仆，等.基于光纤型偏振敏感光学相干层析成像的猪皮烧伤多参量成像分析[J].中国激光，2024,51(3):113-123.
[3] SHPICHKA A,BUTNARU D,BEZRUKOV E A,et al.Skin tissue regeneration for burn injury[J/OL].Stem cell research & therapy,2019,10(1):94[2024-03-12].https://doi.org/10.1186/s13287-019-1203-3.
[4] 谷晶晶.TWEAK/Fn14信号通路调控成纤维细胞细胞外基质影响瘢痕疙瘩发病的机制研究[D].广州：南方医科大学，2022.
[5] BROUGHTON G II,JANIS J E,ATTINGER C E.Wound healing:an overview[J/OL].Plastic and reconstructive surgery,2006,117:1e-S-32e-S[2024-02-13].https://doi.org/10.1097/01.prs.0000222562.60260.f9.
[6] 韩娟.透明质酸与其受体蛋白CD44间的相互作用研究[D].东营：中国石油大学(华东),2016.
[7] 李陈，常克，杨静，等.紫草及紫草制剂的抗炎作用研究进展[J].世界中医药，2018,13(6):1363-1367.
[8] 吴庭辉.紫草素对烧烫伤小鼠皮肤愈合的影响及其分子机制分析[D].阿拉尔：塔里木大学，2023.
[9] 刘林祥，宋红月，牛惠珍.小鼠烫伤模型及其在中草药抗菌作用研究中的初步运用[J].微生物学通报，1989,16(3):157-160.
[10] 刘毅，薛晓东，贾赤宇，等.恒温恒压电烫仪建立小鼠烫伤模型的实验研究[J].兰州医学院学报，2001(1):6-8.
[11] 宋维旭，伍津津，鲁元刚，等.天狼猩红-偏振光检测组织工程皮肤中胶原的观察[J].重庆医学，2007,36(5):450-451,553.
[12] 果秋婷，张小飞，边敏琦，等.基于网络药理学：分子对接探究三黄地榆油治疗烧烫伤的作用机制研究[J].中国药师，2021,24(1):46-53.
[13] 王双勋，李大勇.拔毒生肌散调控Keap1/Nrf2通路促进糖尿病大鼠皮肤溃疡愈合的机制研究[J].中国中医基础医学杂志，2023,29(10):1645-1649.
[14] 陈亚红.瘢痕疙瘩成纤维细胞耐药性和紫草素对瘢痕疙瘩成纤维细胞生长抑制作用的研究[D].上海：上海交通大学，2016.
[15] 杨敏，卢静，蒋学华.透明质酸治疗烧烫伤的系统评价[J].中国药学(英文版),2013,22(3):277-281.
[16] 林影影，邹俊波，何宜航，等.不同提取工艺蛋黄油对小鼠烫伤治疗作用研究[J].亚太传统医药，2016,12(22):12-15.
[17] SHEN C C,SYU W J,LI S Y,et al.Antimicrobial activities of naphthazarins from Arnebia euchroma[J].Journal of natural products,2002,65(12):1857-1862.
[18] 艾静.四种中药单体对孢子丝菌体外抑菌活性的研究[D].长春：吉林大学，2022.
[19] 王杨，陈菲，谢明杰.紫草素对白色念珠菌的抑制作用机制[J].微生物学报，2018,58(10):1817-1825.
[20] 孙丹，傅宏阳，杨晓红，等.紫草素对糠秕马拉色菌作用下HaCaT细胞增殖的影响及其对缺氧诱导因子1α表达的调控[J].临床皮肤科杂志，2022,51(5):271-275.
[21] 李静，武万强，杨靖亚.紫草素可抑制在RAW264.7巨噬细胞和斑马鱼幼虫中由脂多糖诱导的炎症反应[J].甘肃农业大学学报，2023,58(1):38-45.
[22] 郭涛.紫草素对小鼠内毒素血症所致心肌损伤的保护作用及机制研究[D].长沙：中南大学，2022.
[23] 黎珊珊，区岛良，张梅，等.紫草素对糖尿病周围神经病变大鼠的治疗作用及对腺苷酸活化蛋白激酶/核因子E2相关因子2信号通路的影响[J].陕西医学杂志，2023,52(6):683-687.
[24] 郭丽丽.紫草素对H2O2诱导血管内皮细胞损伤的保护和皮肤微血管重建作用的研究[D].阿拉尔：塔里木大学，2023.
[25] 万煜.紫草素对H2O2诱导成纤维细胞损伤的保护以及皮肤胶原重建作用的研究[D].阿拉尔：塔里木大学，2023.
[26] LI Y Y,LU Y J,ZHAO Y C,et al.Deciphering the wound-healing potential of collagen peptides and the molecular mechanisms:a review[J].Journal of agricultural and food chemistry,2024,72(47):26007-26026.
[27] FAN C,XIE Y,DONG Y,et al.Investigating the potential of Shikonin as a novel hypertrophic scar treatment[J/OL].Journal of biomedical science,2015,22(1):70[2024-01-10].https://doi.org/10.1186/s12929-015-0172-9.
[28] KANG T K,LE T T,KIM K A,et al.Roots of Lithospermum erythrorhizon promotes retinal cell survival in optic nerve crush-induced retinal degeneration[J/OL].Experimental eye research,2021,203:108419[2024-03-15].https://doi.org/10.1016/j.exer.2020.108419.
[29] DORSCHNER R A,LEE J,COHEN O,et al.ECRG4 regulates neutrophil recruitment and CD44 expression during the inflammatory response to injury[J/OL].Science advances,2020,6(11):eaay0518[2024-02-13].https://doi.org/10.1126/sciadv.aay0518.
[30] SONG X J,DING F H,LUO W,et al.Knockdown of CD44 inhibits proliferation,migration,and invasiveness in hepatocellular carcinoma cells by modulating CXCR4/Wnt/β-catenin axis[J].Acta biochimica polonica,2023,70(1):117-122.
基本信息:
中图分类号:R285.5
引用信息:
[1]管浩丞,王东阳,武振辉,等.自制紫草抑菌膏对Ⅱ度烧伤小鼠胶原沉积及伤口愈合的影响[J].塔里木大学学报,2025,37(06):35-41.
基金信息:
新疆维吾尔自治区科技厅与兵团科技局联合资助“天山英才”科技创新领军人才培育项目(2023TSYCLJ0057); 新疆生产建设兵团南疆重点产业创新发展支撑计划项目(2021DB023)
2024-09-12
2024
2025-04-17
2025
2
2025-12-15
2025-12-15