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以塔里木盆地药用植物骆驼蓬为研究对象,分离内生细菌,探究其内生细菌多样性并检测抑菌活性,发掘骆驼蓬内生菌物种资源和应用潜力。选取骆驼蓬根、茎、果实进行表面消毒,采用7种培养基进行分离培养,获得72株细菌,经16S rDNA鉴定,分布于7个目、15个科、17个属,其中优势菌属为芽孢杆菌属。以引起果树病害的解淀粉欧文氏菌为靶标,采用平板对峙法和孔碟法对骆驼蓬内生细菌进行抑菌活性筛选,获得9个活性菌株,其中芽孢杆菌属(Bacillus)4株、大洋芽孢杆菌属(Oceanobacillus)、微小杆菌属(Exiguobacterium)、链霉菌属(Streptomyces)、农杆菌属(Agrobacterium)、亮杆菌属(Leucobacter)各1株。本研究的开展为塔克拉玛干沙漠特殊环境药用植物内生菌资源的开发和农用微生物药物的开发奠定了基础。
Abstract：In this study, the endophytic bacteria were isolated, the diversity of endophytic bacteria was investigated, and the antibacterial activity was detected, so as to explore the species resources and application potential of endophytic bacteria in Peganum harmala,a medicinal plant in the Tarim Basin. The roots, stems and fruits of P. harmala were selected for surface disinfection, and 7 kinds of media were used for isolation and culture. 72 strains of bacteria were obtained, which were identified by 16S rDNA and distributed in 7 orders, 15 families, 17 genera, among which the dominant bacteria genus was Bacillus. In this study, we screened the endophytic bacteria of P. harmala by plate and plate methods, and obtained 9 active strains, including 4 Bacillus, 1 Oceanobacillus, 1 Exiguobacterium, 1 Streptomyces, 1 Agrobacterium and 1 Leucobacter. This study laid a foundation for the development of endophytic bacteria resources of medicinal plants in special environment of Taklimakan Desert and the development of agricultural microbial drugs.
[1]VIKALP V,JACOB N,DHRUV K,et al.Potent antitumor effects of a combination of three nutraceutical compounds[J].Scientific Reports,2018,8(1):12163.
[2]文才艺,吴元华,田秀玲.植物内生菌研究进展及其存在的问题[J].生态学杂志,2004,23(2):86-91.
[3]COHEN J.The transplantation of individual rat and guinea-pig whisker papillae[J].Journal of embryology and experimental morphology,1961,9:117-127.
[4]何珊,田志宏.植物内生细菌生物学效应的研究进展[J].安徽农学通报,2020,26(4):20-23.
[5]胡白石.梨火疫病菌的风险分析及检测技术研究[D].南京:南京农业大学,2000.
[6]吴惠秋,徐恩丽,于洋洋,等.梨火疫病菌lux R转录调节因子的克隆与功能分析[J].农业生物技术学报,2013,21(6):99-107.
[7]MANSFIELD J W,GENIN S,MAGORI S,et al.Top 10plant pathogenic bacteria in molecular plant pathology[J].Molecular Plant Pathology,2012,13(6):614-629.
[8]SUN L N,ZHANG Y F,HE L Y,et al.Genetic diversityand characterization of heavy metal-resistant-endophyticbacteria from two copper-tolerant plant pecies on coppermine wasteland[J].Bioresource Technology,2010,101(2):501-509.
[9]封国君.植物内生菌降解二甲四氯的菌株筛选及其降解特性研究[D].南宁:广西大学,2017.
[10]杨鸿儒.西鄂尔多斯荒漠灌木根际细菌多样性和群落结构的研究[D].呼和浩特:内蒙古农业大学,2016.
[11]吕丹,唐克慧,王宇弛,等.毛细管电泳法在药物分析研究中的应用[J].海峡药学,2018,30(218):23-25.
[12]马尔仑.BLAST序列比对脱机移植研究[J].内蒙古师范大学学报(自然科学汉文版),2020,49(4):327-332.
[13]刘新宇,李大皓,李佩瑶.不同培养条件对哈茨木霉菌抑制串珠镰刀菌能力的影响[J].安徽农学通报,2020,26(10):82-85.
[14]杜慧娟.药用植物抗菌内生菌的分离鉴定及其生物学性质研究[D].重庆:重庆大学,2008.
[15]雷艳娟.骆驼蓬内生放线菌多样性及其次级代谢产物初探[D].阿拉尔:塔里木大学,2020.
[16]林丽苗,周庆丰,余国莲,等.副猪嗜血杆菌培养条件优化[J].现代畜牧兽医,2013(7):56-58.
[17]梁慧敏,刘君,王希东,等.梨火疫病抑菌制剂的室内筛选[J].新疆农业科学,2019,56(2):333-344.
[18]ROMERO D,AGUILAR C,LOSICK R,et al.Amyloid fibers provide structural integrity to Bacillus subtilis biofilms[J].Proceedings of the National Academy of Sciences USA,2010,107(2):2230-2234.
[19]ZERIOUH H,VICENTE A,PEREZ-GARCIA A,et al.Surfactin triggers biofilm formation of Bacillus subtilis in melon phylloplane and contributes to the biocontrol activity[J].Environ Microbiol,2014,16(7):2196-2211.
[20]RAHMAN M S,ANO T,SHODA M.Second stage production of it urin a by induced germination of Bacillus subtilis RB14[J].Journal of Biotechnology,2006,125(4):513-515.
[21]陈上,赵瑞丁,双阳,等.一株类芽孢杆菌的分离鉴定及其抗革兰氏阴性菌活性测定[J].微生物学通报,2019,46(10):2665-2672.
[22]赵雅,张岱,杨志辉,等.贝莱斯芽孢杆菌HN-Q-8菌株发酵液稳定性测定及抑菌活性成分分析[J].微生物学通报,2020,47(2):490-499.
[23]李红亚,李术娜,王树香,等.益生芽孢杆菌脂肽类抑菌成分的高效液相色谱-电喷雾串联质谱分析[J].动物营养学报,2017,29(4):1191-1197.
[24]牛世全,李静,张雪莹,等.一株抗黄芪根腐病芽孢杆菌的筛选、鉴定及抑菌物质的初步研究[J].西北师范大学学报(自然科学版),2021,57(2):79-86.
基本信息:
中图分类号:Q939.9
引用信息:
[1]张耀星,朱玉芳,柳耀年,等.骆驼蓬内生细菌多样性与抗菌活性研究[J].塔里木大学学报,2021,33(03):39-47.
基金信息:
国家级大学生创新创业训练计划项目“骆驼蓬内生菌药用代谢产物探索”(201910757051); 中国海洋大学—塔里木大学联合基金项目“塔克拉玛干沙漠稀有放线菌和真菌资源挖掘”(ZHYLH201901)
2021-04-21
2021
2021-06-10
2021
2021-06-10
1
2021-09-15
2021-09-15