| 53 | 0 | 42 |
| 下载次数 | 被引频次 | 阅读次数 |
海藻糖-6-磷酸合成酶(TPS)在植物生长发育及抗逆调控过程中具有重要作用,而其在胡杨(Populus euphratica Oliv.)幼苗期干旱胁迫响应的分子机制尚未明晰。本研究基于比较基因组学分析方法,从胡杨基因组中鉴定出13个PeTPS基因,分布于10条染色体上,其主要源于全基因组复制事件。基因结构与保守结构域分析表明,所有PeTPS基因家族成员均含有8～10个特征性Motif,并具有糖基转移酶结构域(Glyco＿transf＿20)和海藻糖磷酸酶结构域(Trehalose＿PPase)。共线性分析显示,胡杨、灰杨和美洲黑杨之间存在23个TPS基因对,与红皮柳之间存在25个TPS基因对,与拟南芥之间存在16个TPS基因对,表明TPS基因在杨柳科植物内的保守性更高。系统进化分析将胡杨PeTPS基因家族成员划分为5个亚族,其启动子区富含的顺式作用元件主要包括ABA响应元件、干旱诱导的MYB结合位点、非生物胁迫和防御相关响应元件。qRT-PCR表明,在干旱胁迫下PeTPS07、PeTPS09、PeTPS11、PeTPS12和PeTPS13表达上调,PeTPS06表达下调,与转录组数据一致,充分验证了转录组数据的可靠性。本研究为PeTPS基因功能的解析及胡杨抗旱育种工作奠定了理论基础。
Abstract：Trehalose-6-phosphate synthase(TPS) plays pivotal roles in plant growth and stress adaptation. However, through comparative genomics analysis, we systematically identified 13 PeTPS genes distributed across 10 chromosomes, primarily via wholegenome duplication events. Structural characterization revealed that all PeTPS family members harbor 8-10 signature motifs and conserved functional domains, including glycosyltransferase(Glyco＿transf＿20) and trehalose phosphatase(Trehalose＿PPase) domains. Collinearity analysis revealed that there are 23 TPS gene pairs between Populus euphratica and Populus pruinosa as well as Populus deltoides, 25 TPS gene pairs between Populus euphratica and Salix sinopurpurea, and 16 TPS gene pairs between Populus euphratica and Arabidopsis thaliana, indicating high conservation of TPS genes within the Salicaceae family. Phylogenetic classification divided PeTPS genes into five distinct subfamilies. Promoter cis-element profiling identified three functional clusters: phytohormoneresponsive elements(ABA, GA, and SA), stress-responsive elements(drought-induced MYB-binding sites, abiotic stress and defense-related elements), and light-responsive modules. The qRT-PCR results showed that the expression of PeTPS07, PeTPS09, PeTPS11, PeTPS12, and PeTPS13 were upregulated, while the expression of PeTPS06 was downregulated under drought stress, which is consistent with and fully validates the reliability of the transcriptome data. This study establishes a foundation for deciphering PeTPS gene functionality and advancing drought-resilient breeding strategies in desert poplar.
[1] 张丽素，高佳钰，龙健梅，等.黄梁木海藻糖-6-磷酸合酶(TPS)基因家族鉴定与表达分析[J].分子植物育种，2025,23(2):353-361.
[2] 杜姣林，蔺新兰，马豫皖，等.植物海藻糖-6-磷酸合成酶基因研究进展[J].植物科学学报，2023,41(3):411-420.
[3] O’HARA L E,PAUL M J,WINGLER A.How do sugars regulate plant growth and development New insight into the role of trehalose-6-phosphate[J].Molecular plant,2013,6(2):261-274.
[4] NÄGELE T,WECKWERTH W.Mathematical modeling reveals that metabolic feedback regulation of SnRK1 and hexokinase is sufficient to control sugar homeostasis from energy depletion to full recovery[J/OL].Frontiers in plant science,2014,5:365[2025-03-16].https://doi.org/10.3389/fpls.2014.00365.
[5] KIM S J,JEONG D H,AN G,et al.Characterization of a drought-responsive gene,OsTPS1,identified by the T-DNA Gene-Trap system in rice[J].Journal of plant biology,2005,48(4):371-379.
[6] AVONCE N,LEYMAN B,MASCORRO-GALLARDO J O,et al.The Arabidopsis trehalose-6-P synthase AtTPS1 gene is a regulator of glucose,abscisic acid,and stress signaling[J].Plant physiology,2004,136(3):3649-3659.
[7] WANG X L,DU Y,YU D Q.Trehalose phosphate synthase 5-dependent trehalose metabolism modulates basal defense responses in Arabidopsis thaliana[J].Journal of integrative plant biology,2019,61(4):509-527.
[8] SONG J B,MAO H Y,CHENG J,et al.Identification of the trehalose-6-phosphate synthase gene family in Medicago truncatula and expression analysis under abiotic stresses[J/OL].Gene,2021,787:145641[2025-03-16].https://doi.org/10.1016/j.gene.2021.145641.
[9] ZHANG Z Y,CHEN Y,ZHANG J L,et al.Improved genome assembly provides new insights into genome evolution in a desert poplar (Populus euphratica)[J].Molecular ecology resources,2020,20(3):781-794.
[10] SUN J H,XU J D,QIU C,et al.The chromosome-scale genome and population genomics reveal the adaptative evolution of Populus pruinosa to desertification environment[J/OL].Horticulture research,2024,11(3):uhae034[2025-03-16].https://doi.org/10.1093/hr/uhae034.
[11] JIA M Y,WANG Y,JIN H Y,et al.Comparative genomics analysis of the Populus epidermal pattern factor (EPF) family revealed their regulatory effects in Populus euphratica stomatal development[J/OL].International journal of molecular sciences,2024,25(18):10052[2025-03-16].https://doi.org/10.3390/ijms251810052.
[12] TAMURA K,STECHER G,PETERSON D,et al.MEGA6:molecular evolutionary genetics analysis version 6.0[J].Molecular biology and evolution,2013,30(12):2725-2729.
[13] LETUNIC I,BORK P.Interactive tree of life (iTOL) v5:an online tool for phylogenetic tree display and annotation[J].Nucleic acids research,2021,49:293-296.
[14] 韩瑞月，刘晓林，辛同旭，等.黄瓜WIP转录因子家族基因的鉴定及表达分析[J].中国蔬菜，2025(2):44-55.
[15] SUN J H,XU J D,QU W R,et al.Genome-wide analysis of R2R3-MYB transcription factors reveals their differential responses to drought stress and ABA treatment in desert poplar (Populus euphratica)[J/OL].Gene,2023,855:147124[2025-03-16].https://doi.org/10.1016/j.gene.2022.147124.
[16] LIU C Y,HAO J J,QIU M Q,et al.Genome-wide identification and expression analysis of the MYB transcription factor in Japanese plum (Prunus salicina)[J].Genomics,2020,112(6):4875-4886.
[17] 孙梦利，徐子健，王世豪，等.西瓜TPS家族基因的鉴定、分类与分析[J].分子植物育种，2018,16(9):2778-2785.
[18] KOSAR F,AKRAM N A,SADIQ M,et al.Trehalose:a key organic osmolyte effectively involved in plant abiotic stress tolerance[J].Journal of plant growth regulation,2019,38(2):606-618.
[19] CANNON S B,MITRA A,BAUMGARTEN A,et al.The roles of segmental and tandem gene duplication in the evolution of large gene families in Arabidopsis thaliana[J/OL].BMC plant biology,2004,4:10[2025-03-16].https://doi.org/10.1186/1471-2229-4-10.
[20] TUSKAN G A,DIFAZIO S,JANSSON S,et al.The genome of black cottonwood,Populus trichocarpa (Torr.& Gray)[J].Science,2006,313(5793):1596-1604.
[21] VISHAL B,KRISHNAMURTHY P,KUMAR P P.Arabidopsis class II TPS controls root development and confers salt stress tolerance through enhanced hydrophobic barrier deposition[J/OL].Plant cell reports,2024,43(5):115[2025-03-16].https://doi.org/10.1007/s00299-024-03215-w.
[22] ALMEIDA A M,VILLALOBOS E,ARAÚJO S S,et al.Transformation of tobacco with an Arabidopsis thaliana gene involved in trehalose biosynthesis increases tolerance to several abiotic stresses[J].Euphytica,2005,146(1):165-176.
基本信息:
中图分类号:S792.11
引用信息:
[1]龙鑫月,孙健皓,宋童瑞,等.胡杨海藻糖-6-磷酸合成酶(TPS)基因家族鉴定与表达分析[J].塔里木大学学报,2026,38(01):14-23.
基金信息:
新疆生产建设兵团指导性科技计划项目(2024ZD087); 2024年“天池英才”青年博士引进计划项目(524307006); 塔里木大学校长基金博士人才项目(TDZKBS202422、TDZKBS202532)