塔里木大学学报

为明确萌芽前‘冬枣’枝条的含水率及耐寒生理指标对低温胁迫的生理响应,以期为阿拉尔垦区‘冬枣’采集接穗提供理论参考依据。本研究于2024年3月18日—4月15日‘冬枣’枝条萌芽前期,每日采集1 a生枝条,测定分析其含水率、可溶性糖、可溶性蛋白、丙二醛、游离脯氨酸含量的动态变化,结合灰色关联度综合评价。结果表明:2024年阿拉尔垦区‘冬枣’在4月10日芽尖出现绿点,开始逐步进入萌芽期,在4月13日全部萌芽,‘冬枣’枝条的含水率在4月2日—4月10日相较稳定,在4月7日含水率最低,为33.09%。在3月24日—4月10日枝条可溶性糖含量逐步升高,在4月10日枝条可溶性糖达到最高,为11.24%,进入萌芽期,枝条可溶性糖含量明显下降。在3月18日—4月15日枝条可溶性蛋白相对稳定,整体呈下降趋势,在萌芽期4月10日含量最低,为3.11 mg∕g。在4月1日—4月10日枝条丙二醛含量趋于稳定,在4月4日枝条丙二醛含量最低,为0.000 5 mg∕g。在3月29日—4月10日枝条游离脯氨酸含量呈整体下降趋势,变化幅度较小,平均游离脯氨酸含量为1.891 mg∕g。经灰色关联度分析,萌芽前8 d(4月2日—4月10日,平均温度在9.0～16.5℃之间)‘冬枣’枝条含水率和可溶性糖含量整体水平较高且稳定,丙二醛含量极低,可溶性蛋白相对稳定,为‘冬枣’接穗剪取的最佳时期。

To clarify the physiological responses of water content and cold resistance indicators of Ziziphus jujuba Mill. cv. ‘Dongzao' branches before sprouting to low-temperature stress, and to provide a reference basis for the collection of scions in the Alar Reclamation Area. During the pre-budbreak period of Ziziphus jujuba Mill. cv. ‘Dongzao' from March 18 th to April 15 th, 2024, one-year-old branches were collected daily in this research. Moisture content, soluble sugars, soluble proteins, malondialdehyde(MDA), and free proline were measured and analyzed for their response to external low-temperature conditions. The cold resistance of the branches was comprehensively evaluated in combination with the grey relational degree. The results indicated that on April 10 th,2024, green spots appeared on the tips of the Ziziphus jujuba Mill. cv. ‘Dongzao' in the Alar, and they gradually entered the germination period. All full sprouting achieved by April 13 th. Branch moisture content remained relatively stable from April 2 th to April 10 th, and reaching a minimum of 33. 09% on April 7 th. Soluble sugar content gradually increased from March 24 th, peaking at 11. 24% in the branches on April 10 th before decreasing markedly upon sprouting. Soluble protein levels exhibited overall stability but showed a declining trend; the lowest value recorded was 3. 11 mg∕g on April 10 th. In the branches, MDA content stabilized between April 1 th and April 10 th, with a minimum of 0. 000 50 mg∕g observed on April 4 th. From March 29 th to April 10 th, the content of free proline in the branches showed an overall downward trend with a relatively small change range, and the average content of free proline was 1. 891 mg∕g. Through Ggrey relational analysis revealed that the comprehensive evaluation of cold resistance indicators was highest during the eight days before germination( Apri 2-10 th; average temperature:9. 0-16. 5 ℃). The overall water content and soluble sugar levels were relatively high and stable, the MDA concentrations was extremely low, and relatively stable soluble protein levels throughout this timeframe. This study provides a scientific theoretical basis for the optimal scion collection period for ‘Dongzao'.