塔里木大学学报

本试验以竹节秋海棠叶片为外植体材料进行组织培养，研究不同消毒时间对外植体灭菌效果的影响；不同浓度的激素配比对竹节秋海棠初代不定芽诱导、继代不定芽增殖以及不定根诱导情况的影响；不同基质配比对驯化移栽的影响，以期建立适合竹节秋海棠的组培快繁技术体系。结果表明，最适合竹节秋海棠叶片灭菌的方式为75%的乙醇消毒30 s后用2%的次氯酸钠溶液消毒5 min,其成活率为100%;MS+1.0 mg/L 6-BA+0.6 mg/L IBA适合不定芽诱导，其诱导率为100%;MS+0.1 mg/L 6-BA+1.0 mg/L IBA适合不定芽增殖扩繁，其增殖系数为1.40;1/2MS+0.2 mg/L NAA适合不定根生长，1/2MS+0.2 mg/L IBA适合不定根伸长，其生根率皆为100%;适合驯化移栽的基质配比为草炭∶蛭石=1∶1,其成活率为100%。

In this experiment, tissue culture was conducted on the leaves of Begonia maculata Raddi, and the effect of different disinfection time on the sterilization effect of the explants was studied. Effects of different concentrations of hormones on adventitious bud induction in the first generation, adventitious bud proliferation in subsequent generations and adventitious root induction in Begonia maculata were experimented. The effects of different substrate ratio on transplanting in order to establish the system of rapid propagation technique of Begonia maculata Raddi in tissue culture.The results showed that the survival rate of leaves was 100% after disinfection with 75% ethanol for 30 s and 2% sodium hypochlorite solution for 5 min. MS+1.0 mg/L 6-BA+0.6 mg/L IBA was suitable for adventitious bud induction, and the induction rate was 100%. MS+0.1 mg/L 6-BA+1.0 mg/L IBA was suitable for adventitious bud proliferation and propagation, and the proliferation coefficient was 1.40. Then the 1/2MS+0.2 mg/L NAA was suitable for adventitious root growth, and 1/2MS+0.2 mg/L IBA was suitable for adventitious root elongation, both rooting rates were 100%. The survival rate of the substrate suitable for acclimation and transplanting was 100% on the conditions of turf∶vermiculite = 1∶1.