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通过以人肝肿瘤细胞Hep G2为体外研究模型,采用了常见的电化学方法以及借助显微镜技术评估了甘草酸和甘草黄酮对它的影响,发现甘草酸在培养基中浓度低于2.0 g/L时,对肿瘤细胞的抑制效应比较小,当浓度扩大(大于2.0 g/L)时,抑制效应明显增大,细胞凋亡数目变化显著,并且在甘草酸浓度达到4.0 g/L之前,呈明显的浓度依赖性。而相同条件下甘草黄酮的抑制效应不大明显。通过本次研究,可以看出,甘草酸表现出了对肿瘤细胞Hep G2具有较明显的抑制作用。可以预见,作为一种比较常见的天然产物成分,它将在防癌抗癌药物的开发和研制当中得到人们的重视和应用。
Abstract：The human liver tumor cells,Hep G2 were selected as a model to assess the effects of glycyrrhizic acid( GA) and flavonoids of glycyrrhiza on them by using a microscopy and the classical electrochemical method in vitro. It was found that the inhibition efficiencies of GA to Hep G2 were more significant than those of flavonoids of glycyrrhiza in the same concentration. And the inhibition efficiencies to the cells showed very insignificant when the concentration of GA was less than 2. 0 g / L in the culture medium. While the concentration was up to more than 2. 0 g / L,the number of the cell apoptosis augmented markedly. And it presented an obvious concentration- dependence relationship till the concentration of GA reached up to 4. 0 g / L. It is confirmed that glycyrrhizic acid,a common main component in Radix Glycyrrhizae,can inhibit the growth and development of tumor cells Hep G2 obviously in this study. And it can be predicted that GA will attract more and more attention in the development of anti- tumor medicine in the future.
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基本信息:
中图分类号:R735.7
引用信息:
[1]姜项福,刘文杰,白红进.甘草酸和甘草黄酮与人肝癌细胞(HepG2)作用的电化学研究[J].塔里木大学学报,2014,26(01):17-23.
基金信息:
塔里木大学硕士基金项目(TDZKSS201107);; 国家自然科学基金(71067012)
2014-03-15
2014-03-15