| 3,927 | 45 | 161 |
| 下载次数 | 被引频次 | 阅读次数 |
石蜡切片的制作是组织学和病理学中常用的一种方法。常规制作切片的方法,往往达不到满意的效果。本文根据作者十年的制片经验,摸索其药品的配制,对实验步骤和操作方法等细节方面进行了改良,大大的提高了切片的染色效果和整体质量,明显降低脱片率(低于5%)。
Abstract:The process,embed and section of paraffin section are usually performed by a histology and pathology laboratory.While hand processing can be performed according to the routine procedure,the results may show marked variation in slide quality.In this text,the author improved the staining effectiveness and quality of paraffin section which is stabilized for long-term storage,according to improving mixed reagent and operational means during her experimental career of more than ten years.
[1]杜辉,王树迎.组织切片制作中的点滴经验[J].解剖学杂志,2004,27(2):165.
[2]路健,王玲.石蜡切片制作中的问题与解决[J].基础医学教育,2001,3:263.
[3]毕振华.冰冻切片染色方法的改良[J].临床与实验病理学杂志,1994,10(4);289.
[4]李文平,王基清.纱布覆盖法防止组织切片中片染卷脱[J].人民军医.1996,243(6):57.
[5]宋平,张世华.HE染色质量差的原因及处理方法[J].诊断病理学杂志,1995,2(1):52.
基本信息:
中图分类号:S852
引用信息:
[1]廖秋萍,石长青,饶丽娟.石蜡切片制片技术的探讨[J].塔里木大学学报,2006(01):69-71.