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小肠结肠炎耶尔森菌为嗜冷菌，能够在低温、低氧的环境下生存，是一类食源性病原体，能够感染人及多种动物。本研究旨在了解新疆阿克苏地区部分集约化奶牛场小肠结肠炎耶尔森菌污染情况，在2022年期间采集阿克苏地区部分奶牛养殖场奶牛粪便、原料乳、饲草、采乳设备、环境样品，共计240份。采用形态学观察、生化鉴定、分子生物学的方法对小肠结肠炎耶尔森菌进行分离与鉴定。结果表明共分离出23株小肠结肠炎耶尔森菌，3个县市均分离出该菌，且分离率相近，均约为9%。本试验能为阿克苏地区部分集约化奶牛场小肠结肠炎耶尔森菌的防治、杀灭提供理论依据和参考价值。
Abstract：Yersinia enterocolitica, a psychrotrophic bacteria and food-borne pathogen, has the ability to survive in low temperature, low oxygen environments, and infect both humans and various. This study aimed to investigate the contamination of Yersinia enterocolitica in the small intestine within intensive dairy farms in Aksu. A total of 240 samples including cow feces, raw milk, forage grass, milking equipment, and environmental samples were collected from these dairy farms in Aksu during 2022. Yersinia enterocolitica was isolated and identified through morphological observation, biochemical identification and molecular biology techniques. The results revealed the isolation of 23 strains of Yersina enterocolitica across three different counties and cities, with a separation rate of approximately 9% that was consistent across the various areas investigated. The findings of this study can serve as a theoretical basis and provide valuable reference for the prevention and eradication of Yersinia enterocolitica in intensive dairy farms in Aksu.
[1] 祁腾，赵雯霞，李文博，等.四川省鼠疫疫源地内啮齿动物携带小肠结肠炎耶尔森菌调查[J].预防医学情报杂志，2022,38(5):599-602,606.
[2] 于恩庶.中国小肠结肠炎耶尔森氏菌病研究进展[J].中华流行病学杂志，2000,21(6):453-455.
[3] MACDONALD E,HEIER B T,NYG?RD K,et al.Yersinia enterocolitica outbreak associated with ready-to-eat salad mix,Norway,2011[J].Emerging infectious diseases,2012,18(9):1496-1499.
[4] YOUNIS G,MADY M,AWAD A.Yersinia enterocolitica:prevalence,virulence,and antimicrobial resistance from retail and processed meat in Egypt[J].Veterinary world,2019,12(7):1078-1084.
[5] ROSNER B M,STARK K,WERBER D.Epidemiology of reported Yersinia enterocolitica infections in Germany,2001—2008[J].BMC public health,2010,10:337.
[6] LE GUERN A S,MARTIN L,SAVIN C,et al.Yersiniosis in France:overview and potential sources of infection[J].International journal of infectious diseases,2016,46:1-7.
[7] 关乃瑜，夏爽，赵丽丽，等.断奶仔猪源小肠结肠炎耶尔森氏菌的分离鉴定及其致病性研究[J].中国预防兽医学报，2015,37(9):679-682.
[8] HANIFIAN S,KHANI S.Prevalence of virulent Yersinia enterocolitica in bulk raw milk and retail cheese in northern-west of Iran[J].International journal of food microbiology,2012,155(1/2):89-92.
[9] 郑宇.牛羊小肠结肠炎耶尔森氏菌流行病学调查及其检测方法的建立[D].长春：吉林大学，2021.
[10] 袁磊.原料奶中嗜冷菌的潜在危害研究：基于腐败特性及其生物被膜形成的角度[D].杭州：浙江大学，2020.
[11] LIANG J R,DUAN R,XIA S L,et al.Ecology and geographic distribution of Yersinia enterocolitica among livestock and wildlife in China[J].Veterinary microbiology,2015,178(1/2):125-131.
[12] 陈邬锦，王鹏.中国小肠结肠炎耶尔森菌流行现状及其研究进展[J].中国人兽共患病学报，2015,31(4):380-384.
[13] DONNELLY C W.Concerns of microbial pathogens in association with dairy foods[J].Journal of dairy science,1990,73(6):1656-1661.
[14] 吴鑫，章志超，刘德，等.2018—2019年南昌市市售生鲜肉中小肠结肠炎耶尔森氏菌污染情况分析[J].食品安全质量检测学报，2020,11(13):4214-4218.
[15] BACKHANS A,FELLSTR?M C,LAMBERTZ S T.Occurrence of pathogenic Yersinia enterocolitica and Yersinia pseudotuberculosis in small wild rodents[J].Epidemiology & infection,2011,139(8):1230-1238.
基本信息:
中图分类号:S858.23
引用信息:
[1]候梦哲,李雪娇,郝斌,等.阿克苏地区集约化奶牛场小肠结肠炎耶尔森菌污染情况调查[J].塔里木大学学报,2024,36(03):30-35.
基金信息:
新疆生产建设兵团重点领域科技攻关计划项目(2019AB029)
2024-09-15
2024-09-15