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为了探究紫运托峰紫草抑菌膏对小鼠Ⅱ度烧烫伤创面的治疗效果，评估其对皮肤组织恢复及转化生长因子-β(TGF-β)和碱性成纤维细胞生长因子(bFGF)表达的影响。本试验使用自制造创仪在昆明小鼠背部制造Ⅱ度烧烫伤创口，通过HE染色法和天狼星红染色法观察创口恢复情况，使用qPCR技术和免疫荧光法检测相关因子TGF-β和bFGF的表达，并以市售常见的烧伤膏作为阳性对照，对紫运托峰紫草抑菌膏的治疗效果进行研究。结果表明：紫运托峰紫草抑菌膏对小鼠Ⅱ度烧烫伤背部创面皮肤的恢复具有显著效果。通过HE染色和天狼星红染色观察，紫运托峰紫草抑菌膏组的创面皮肤修复效果优于模型组(阴性对照)和烧伤膏组(阳性对照)。qPCR和免疫荧光检测结果显示，紫运托峰紫草抑菌膏能够上调TGF-β和bFGF的表达，且效果优于烧伤膏组(P<0.05),说明紫运托峰紫草抑菌膏能有效促进TGF-β和bFGF的表达，加速烧烫伤小鼠创面愈合。
Abstract：In order to investigate the therapeutic effect of Ziyuntuofeng lithospermum-containing bacteriostatic ointment on the wounds of mice with Ⅱ degree burns and to evaluate the effect of the ointment on the skin tissue recovery and the expression of transforming growth factor-β(TGF-β) and basic fibroblast growth factor(bFGF). In this experiment, a self-made device was used to create II degree burn wounds on the back of Kunming mice. The wound recovery was observed by HE staining and Sirius red staining, and the expressions of TGF-β and bFGF were detected by qPCR and immunofluorescence. The therapeutic effect of Ziyuntuofeng lithospermum-containing bacteriostatic ointment was studied by using commercially common burn ointment as a positive control. The results showed that Ziyuntuofeng lithospermum-containing bacteriostatic ointment had remarkable effect on the recovery of back wound skin of mice burned by degree Ⅱ. According to HE staining and Sirius red staining, the wound healing effect of Ziyuntuofeng lithospermum-containing bacteriostatic ointment group was better than that of model group(negative control) and burn ointment group(positive control). The results of qPCR and immunofluorescence detection showed that Ziyuntuofeng lithospermum-containing bacteriostatic ointment group could up-regulate the expression of TGF-β and bFGF, and the effect was significantly better than that of burn ointment group(P<0.05), indicating that the Ziyuntuofeng lithospermum-containing bacteriostatic ointment could effectively promote the expression of TGF-β and bFGF, and accelerate the wound healing of burned mice.
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基本信息:
中图分类号:R285.5
引用信息:
[1]徐孟龙,朱坤龙,管浩丞,等.紫运托峰紫草抑菌膏对小鼠Ⅱ度烧烫伤的影响[J].塔里木大学学报,2025,37(05):1-8.
基金信息:
新疆维吾尔自治区科技厅与兵团科技局联合资助“天山英才”培养计划科技创新领军人才培育项目(2023TSYCLJ0057); 新疆生产建设兵团南疆重点产业创新发展支撑计划项目(2021DB023)
2024-09-13
2024
2025-09-28
2024-11-08
2024
1
2025-10-15
2025-10-15