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本实验拟采用Plackett Burman(PB)试验设计筛选出影响Bacillus axarquiensis TUBP1产抗菌蛋白关键因素,并采用响应面设计中Box-Behnken(BB)实验设计对其产抗菌蛋白最佳配比进行优化。本文使用Plackett Burman设计对影响B.axarquiensis TUBP1产抗菌蛋白的8个因素进行筛选,得到影响抗菌蛋白产量显著的因素为葡萄糖、蛋白胨和发酵p H。在此基础上进一步采用Box-Behnken设计对这3个显著因素的最佳配比进行优化,得到葡萄糖、蛋白胨、p H最佳配比为葡萄糖2.53%、蛋白胨1.76%和p H7.17,与优化前相比蛋白产量提高了34.55%。
Abstract：To improve and optimize antifungal protein production by a newly isolated B. axarquiensis,Plackett- Burman( PB) design and Box- Behnken design( BB) were adopted in culture conditions,the PB design was undertaken to evaluate the effects and Box- Behnken design was used to optimize the critical internal factors. The results showed that,the peptone,glucose and p H were the key factors,the optical concentration of the variables were determined as glucose at 2. 53%,peptone at 1. 76% and p H at 7. 17. Protein yield increased by 34. 55% compared with before optimization.
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基本信息:
中图分类号:TQ920.6
引用信息:
[1]马江锋,曾红.响应面法优化棉花黄萎病拮抗菌Bacillus axarquiensis TUBP_1产抗菌蛋白发酵条件研究[J].塔里木大学学报,2016,28(01):5-13.
基金信息:
国家自然科学基金(31260013);; 新疆生产建设兵团博士基金(2013BB006);; 塔里木大学校长博士基金(TDZKBS201206)
2016-03-15
2016-03-15