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猪伪狂犬病是由伪狂犬病病毒(PRV)引起的一种高度接触性猪传染病，该病能引起仔猪死亡和怀孕母猪流产等，给养殖业带来巨大的经济损失。除了典型的临床症状，PRV还可以引起猪群免疫抑制，导致继发感染，增加治疗费用，同时使猪只摄食量与饲料报酬下降，饲料损耗增加，上市延迟。为了减少新疆地区养猪业的损失，本试验围绕猪伪狂犬病毒的检测开展研究，为猪伪狂犬病的防控提供基础数据。采集新疆地区某猪场病死哺乳仔猪和保育仔猪的脑、肺脏、淋巴结等组织病料，提取总DNA,参考国家标准GB/T 18641—2018《伪狂犬病诊断方法》,设计扩增猪伪狂犬gE基因的引物，采用gE的国标引物对病料中伪狂犬的gE基因进行鉴定，扩增产物大小为368 bp,克隆鉴定后测序，序列分析表明该PRV-gE基因序列与国外的PRV-gE基因序列的同源性为97.7%～97.9%,与国内的PRV-gE基因序列的同源性为99.4%～99.9%,说明该PRV-gE基因序列的伪狂犬毒株为中国流行毒株，本研究为伪狂犬病的净化和疫苗的选择提供参考。
Abstract：Porcine pseudorabies is a highly contagious disease of pigs caused by the pseudorabies virus(PRV). It has the potential to cause death in young pigs and abortion in pregnant sows, resulting in significant economic losses for the pig farming industry. In addition to the typical clinical symptoms, PRV can also cause immunosuppression in pig populations, leading to secondary infections, increased treatment costs, and a reduction in feed intake and feed conversion efficiency. Furthermore, there is an increase in feed wastage and a delay in marketing. In order to reduce the economic losses incurred by the pig farming industry in Xinjiang, this study conducted research on the detection of pseudorabies virus, thereby providing the basic data required for the control of pseudorabies. Tissues from the brains, lungs, and lymph nodes of deceased lactating and weaned piglets were obtained from Xinjiang a certain pig farm and subjected to total DNA extraction. The gE gene of pseudorabies virus was identified using primers based on the national standard GB/T 18641—2018 Diagnostic method for pseudorabies, and the amplified product was 368 bp in size. The cloned and identified product was sent for sequencing, and the sequence analysis demonstrated that the PRV-gE gene sequence exhibited an identity of 97.7%-97.9% with foreign PRV-gE gene sequences and 99.4%-99.9% with domestic PRV-gE gene sequences. This indicated that the PRV-gE gene sequence of the pseudorabies virus strain was a Chinese prevalent strain. The findings of this study provide a reference point for the eradication of pseudorabies and the selection of an appropriate vaccine.
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基本信息:
DOI：
中图分类号:S858.28
引用信息:
[1]刘淑华,刘祥杰,陈鑫等.新疆地区某猪场猪伪狂犬gE基因的检测与分析[J].塔里木大学学报,2025,37(02):1-7.
基金信息:
新疆生产建设兵团科技特派员创新创业项目(2018CB037)